GEL ELECTROPHORESIS

Electrophoresis is a technique that enables separation and analysis of charged molecules in an electric field. Gel electrophoresis is most commonly used for separation and purification of proteins and nucleic acids that differ in size, charge, or conformation. The gel is composed of polyacrylamide or agarose. Polyacrylamide is preferred for proteins and smaller DNA fragments.

GEL ELECTROPHORESIS

PROTEIN ANALYSIS BY PAGE

ICH Q6B requires electrophoretic patterns for the analysis of Biopharmaceuticals.

  • Protein size determination
  • Protein identification
  • Impurity profiling
  • Aggregate analysis

PROTEIN ANLAYSIS BY PAGE- CASE STUDIES

Protein size determination of mAb sample

Protein size determination of mAb sample

PROTEIN ANLAYSIS BY PAGE- CASE STUDIES

Aggregate analysis

PROTEIN ANALYSIS BY PAGE AT CBA

 

PROTEIN ANALYSIS BY PAGE 

Sample requirement

Amount- 1 ml (0.1-1 mg/ml)

Deliverables

Gel Images (Staining by CBB) 

Information required from client

  • Approximate Mol. Wt of Protein
  • Buffer composition (in case of formulation)

SERVICES OFFERED BY CBA

  • Protein Intact Mass Analysis using HRMS
  • Peptide Mapping using HRMS
  • Post Translational Modifications Analysis using HRMS
  • Host Cell Protein Impurity Identification using HRMS
  • Host Cell Protein Impurity Profiling using SDS-PAGE
  • Secondary Structure Analysis using far-UV CD Spectroscopy
  • Tertiary Structure Analysis using near-UV CD Spectroscopy
  • Tertiary Structure Analysis using Fluorescence Spectroscopy
  • Higher Order Structure Analysis using Fluorescence Spectroscopy
  • Secondary Structure Analysis using FTIR Spectroscopy
  • Higher Order Structure Analysis using DSC
  • Aggregate/Multimers Analysis using SEC-MALS
  • Protein Protein Interaction Analysis using SPR
  • Biosimilarity assessment Project

SERVICES OFFERED BY CBA

Dr. Smita Kale

PI- Center for BioPharma Analysis

Venture Center

100, NCL Innovation Park, Dr. Homi Bhabha Road, Pune – 08

Phone: 020-2586-5877 | Mobile: +91-9923147592

Email: smita@venturecenter.co.in

Mr. Yashodhan Shende

Business Development Executive

Venture Center

100, NCL Innovation Park, Dr. Homi Bhabha Road, Pune – 08

Phone: 020-2586-5877 | Mobile: +91-7755920096 

Email: yashodhan@venturecenter.co.in

For more information, please contact:

Website: https://bioanalysis.in/