GEL ELECTROPHORESIS
Electrophoresis is a technique that enables separation and analysis of charged molecules in an electric field. Gel electrophoresis is most commonly used for separation and purification of proteins and nucleic acids that differ in size, charge, or conformation. The gel is composed of polyacrylamide or agarose. Polyacrylamide is preferred for proteins and smaller DNA fragments.
PROTEIN ANALYSIS BY PAGE
ICH Q6B requires electrophoretic patterns for the analysis of Biopharmaceuticals.
- Protein size determination
- Protein identification
- Impurity profiling
- Aggregate analysis
PROTEIN ANLAYSIS BY PAGE- CASE STUDIES
Protein size determination of mAb sample
PROTEIN ANLAYSIS BY PAGE- CASE STUDIES
Aggregate analysis
PROTEIN ANALYSIS BY PAGE AT CBA
| PROTEIN ANALYSIS BY PAGE | |
| Sample requirement | Amount- 1 ml (0.1-1 mg/ml) |
| Deliverables | Gel Images (Staining by CBB) |
| Information required from client |
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