PROTEIN ESTIMATION
Protein estimation is required to determine total protein content in a sample.
BRADFORD ASSAY- PRINCIPLE
The Bradford protein assay is used to determine the concentration of the total protein in a sample. Under acidic conditions, the dye Coomassie Brilliant Blue G-250 binds to the protein and consequently is converted from red form to a stable blue form with the maximum absorbance at 595nm. Thus, the formation of this protein-dye complex can be quantified by measuring the absorbance at 595nm using spectrophotometer or microplate reader to calculate the protein concentration in the sample by comparing the value with the standard curve generated using protein standards.
BRADFORD ASSAY – STANDARD CURVE
Bradford standard curve 1.0mg/mL bovine serum albumin used as standard.
PROTEIN ESTIMATION AT CBA
Protein Estimation by Bradford assay
Sample requirement
Amount- 0.1-0.5 mL
Samples should not have high salt content
Deliverables
Five concentrations of BSA in triplicates; Samples will be prepared in triplicates; Results will be averaged and reported
Information required from Client
Buffer composition (in case of formulation)
CONTACT INFORMATION |
SERVICES OFFERED BY CBA
| •Protein Intact Mass Analysis using HRMS
•Peptide Mapping using HRMS •Post Translational Modifications Analysis using HRMS •Host Cell Protein Impurity Identification using HRMS •Host Cell Protein Impurity Profiling using SDS-PAGE •Secondary Structure Analysis using far-UV CD Spectroscopy •Tertiary Structure Analysis using near-UV CD Spectroscopy •Tertiary Structure Analysis using Fluorescence Spectroscopy •Higher Order Structure Analysis using Fluorescence Spectroscopy •Secondary Structure Analysis using FTIR Spectroscopy •Higher Order Structure Analysis using DSC •Aggregate/Multimers Analysis using SEC-MALS •Protein Protein Interaction Analysis using SPR •Biosimilarity assessment Project |
Dr. Smita Kale
PI- Center for BioPharma Analysis Venture Center 100, NCL Innovation Park, Dr. Homi Bhabha Road, Pune – 08 Phone: 020-2586-5877 | Mobile: +91-9923147592 Email: smita@venturecenter.co.in Mr. Yashodhan Shende Business Development Executive Venture Center 100, NCL Innovation Park, Dr. Homi Bhabha Road, Pune – 08 Phone: 020-2586-5877 | Mobile: +91-7755920096 Email: yashodhan@venturecenter.co.in For more information, please contact: Website: https://bioanalysis.in/ |